V-1 Laboratory for Diagnostics
Laboratory for Diagnostics (V-1) is the central laboratory of the Veterinary Institute Vinkovci, with a long tradition of providing laboratory and field services of diagnostics of animal diseases.
Our customers are primarily Ministry of Agriculture. In their annual decrees (Decree on measures for the protection of animals from infectious and parasitic diseases and their financing or National Programs) and based on the Act on Veterinary Services, the Ministry determines the schedule and type of samples submitted to the laboratory. In addition to the Ministry, our customers are veterinary organisations and other legal entities and individuals.
In the course of 2007, the Laboratory moved into the refurbished premises on the first floor of the building and continued its ordinary operations and introduction of new laboratory methods.
In the course of 2006 and 2007, the preparations were in progress for accreditation according to standard HRN EN ISO/IEC 17025:2005. One of the guarantees of the success of all the changes in our method of work and the quality of our laboratory test results is our classification into the Register of accredited laboratories according to standard HRN EN ISO/IEC 17025:2005: General requirements for the competence of testing and calibration laboratories by the Croatian Accreditation Agency.
V-1 Laboratory has until now accredited four methods:
1. Isolation and identification of motile Salmonella spp. (up to group “O”) from animal faeces and eggs according to HRN EN ISO 6579:2003/Corr. 1:2008;
2. Artificial ingestion for diagnostics of trichinellosis in meat of animals grown for food and wild game according to Commission Regulation (EC) No. 2075/2005;
3. Detection of specific antibodies on CSF virus (ELISA) according to OIE Manual from 2008;
4. Detection of specific antibodies for bovine and swine brucellosis and brucellosis in sheep and goats (Rose-Bengal test) according to OIE Manual from 2009.
In addition to the foregoing methods, V-1 Laboratory is also using other standardized methods, relying on the guidance of the International Office for Epizooties (OIE).
Some of the most important standardized methods are:
1. Enzootic bovine leucosis (EBL) – detection of antibodies in blood and milk (ELISA);
2. Infectious bovine rhinotracheitis (IBR)/infectious pustular vulvovaginitis (IPV) – detection of antibodies in blood (ELISA);
3. Aujeszky’s disease – detection of antibodies in blood (ELISA);
4. Brucellosis in sheep (Brucella ovis infection) – detection of antibodies in blood (ELISA);
5. Mastitis – bacteriological tests of udder secretion samples;
6. Antibiogram – determination of bacteria susceptibility to active antimicrobial substances;
7. Rabies – detection of rabies virus (direct immunofluorescence).
In addition to the foregoing, Laboratory employees conduct field visits when called, perform autopsies on dead animals in order to determine the cause of death and also take samples for laboratory tests (bacteriological, parasitological, pathohistological, etc.).
The Laboratory is appointed by the Ministry of Agriculture – Directorate for Food Safety and Quality the reference laboratory for trichinellosis for artificial indigestion method.
Laboratory for Diagnostics has three veterinary medicine doctors (three PhDs) and three veterinary technicians.
The Laboratory is focused on permanent improvements of its operations through regular external and internal training courses of its staff and regular controls of the sample test results.
Our successful work until now is a guarantee of our quality and compliance with our clients’ requirements.
Laboratory employees:
Marin Periškić, PhD, Laboratory Manager
Davor Balić, PhD, Deputy Manager
Marica Lolić, PhD, laboratory diagnostician
Marina Bačić, laboratory technician
Želimir Smuk, laboratory technician
Željko Rajković, laboratory technician
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